The Wyoming Highway Patrol operates in four distinct Districts throughout the state - commanded by a State Trooper at the rank of Captain. Each of the four Districts are comprised of individual Troops. Each Troop is managed by a State Trooper at the rank of Lieutenant.

Wyoming State Troopers are assigned to a specific Troop in which they primarily patrol.  However, all Wyoming State Troopers have statewide jurisdiction to perform law enforcement duties.  State Troopers often travel throughout the state of Wyoming in performance of their normal duties, to assist with investigations, to participate in training or public events, or to take part in special enforcement details.

Headquarters for the Wyoming Highway Patrol is located in Cheyenne, WY.  Much of the WHP Executive Command Staff, Equipment & Technology staff, Training & Education staff, and the WHP dispatch center operate out of the state capital.